Cod Uracil-DNA Glycosylase (Cod UNG) from Atlantic Cod is the only commercially available UNG enzyme that is completely and irreversibly inactivated by moderate heat treatment. The enzyme is produced in a recombinant E. coli (ung-) strain that contains a modified Cod UNG gene.
Main advantages with Cod UNG
- Completely and irreversibly inactivated at 55°C
- Use of Cod UNG makes contamination control possible in RT-PCR
- Does not degrade PCR product post-PCR. This makes downstream use of the PCR product possible
- High purity enzyme, tested free of contaminating nucleases