Heat&Run Genomic DNA removal kit FAQ

  1. Is H&R compatible with RNase inhibitor?
  2. Is H&R compatible with RNAlater (Life) and similar reagents?
  3. Can RNA treated with H&R be stored?
  4. Can H&R be used together with any reverse transcription kit?
  5. What sources of RNA can be used for H&R cleanup?
  6. Can RNA dissolved in TE buffer be used for H&R cleanup?
  7. What kind of RNA isolation is recommended for RNA being treated with H&R?

Is H&R compatible with RNase inhibitor?

Yes – RNase inhibitor does not affect the performance of the H&R kit.


Is H&R compatible with RNAlater (Life) and similar reagents?

Not tested


Can RNA treated with H&R be stored?

Yes – but beware that Mg remains in the solution after H&R treatment, which might reduce the long-term stability of RNA. If RNA storage after treatment is desired, add EDTA to a final concentration of 3mM after H&R treatment.


Can H&R be used together with any reverse transcription kit?

Yes – However some Mg remains in the RNA after H&R clean-up. In some cases this can interfere with RT performance. If this is the case, simply add EDTA to a final concentration of 3 mM prior to RT.


What sources of RNA can be used for H&R cleanup?

All sources of RNA are compatible with H&R. Successful gDNA removal without detectable loss of RNA has been shown in RNA preps from several species from bacterial, animal and plant.


Can RNA dissolved in TE buffer be used for H&R cleanup?

No – H&R is dependent on Mg to function, thus TE buffer will reduce the activity of the kit.


What kind of RNA isolation is recommended for RNA being treated with H&R?

Any RNA isolation method is suitable. However, avoid elution of / dissolving RNA using TE buffer.