- Is H&R compatible with RNase inhibitor?
- Is H&R compatible with RNAlater (Life) and similar reagents?
- Can RNA treated with H&R be stored?
- Can H&R be used together with any reverse transcription kit?
- What sources of RNA can be used for H&R cleanup?
- Can RNA dissolved in TE buffer be used for H&R cleanup?
- What kind of RNA isolation is recommended for RNA being treated with H&R?
Yes – RNase inhibitor does not affect the performance of the H&R kit.
Yes – but beware that Mg remains in the solution after H&R treatment, which might reduce the long-term stability of RNA. If RNA storage after treatment is desired, add EDTA to a final concentration of 3mM after H&R treatment.
Yes – However some Mg remains in the RNA after H&R clean-up. In some cases this can interfere with RT performance. If this is the case, simply add EDTA to a final concentration of 3 mM prior to RT.
All sources of RNA are compatible with H&R. Successful gDNA removal without detectable loss of RNA has been shown in RNA preps from several species from bacterial, animal and plant.
No – H&R is dependent on Mg to function, thus TE buffer will reduce the activity of the kit.
Any RNA isolation method is suitable. However, avoid elution of / dissolving RNA using TE buffer.