ArcticZymes AS is pleased to announce the launch of Cod UNG Glycerol and Triton FREE and HL-dsDNase Triton FREE. This launch is a continuing step towards maintaining a completely EU REACH compliant product portfolio after Triton X-100 becomes subject to authorisation in January 2021. The convenient glycerol-free formulation is suitable for lyophilization and automated processes. Cod UNG Glycerol and Triton FREE and HL-dsDNase Triton FREE has been developed and manufactured under the same strict quality guidelines we apply to all our enzymes in compliance with ISO 13485. Both enzymes are now available in the webshop and in bulk for our commercial customers.
ArcticZymes AS is pleased to announce the launch of its 1st Ligase in the market. ArcticZymes’ T4 DNA Ligase has been developed and manufactured under the same strict quality guidelines we apply to all of our enzymes in compliance with ISO 13485. T4 DNA Ligase catalyses the formation of phosphodiester bonds between juxtaposed 5’ phosphate and 3’ hydroxyl termini in duplex DNA or RNA.
ArcticZymes AS attention to quality is demonstrated in the alignment of production methodologies with our thorough quality control test and assays. Our rigorous quality control assays test for dsDNA endonuclease, ssDNA endonuclease, dsDNA exonuclease, ssDNA exonuclease, RNase, as well as E. coli gDNA. Additional QC testing may be offered on customer request.
Our introductory pack size is 250,000 Units. T4 DNA Ligase is also available in custom pack sizes and concentrations upon request.
T4 DNA Ligase is adaptable to DNA, RNA and some DNA/RNA hybrids. Applications include: joining restriction digested dsDNA (both blunt and cohesive ends), joining double-strand oligonucleotide linkers and adapters to dsDNA, and applications based on nick-repair and splint ligation.
A paper recently published in Letters in Applied Microbiology describes advantages of using ArcticZymes’ PCR Decontamination Kit in master mix clean-up prior to PCR in microbiome analysis applications.
In molecular diagnostics and applied sciences, a current trend is to migrate from searching for a set of predefined microorganisms to identifying and quantifying all microorganisms present in the sample, known as the microbiome. Analysis of microbiomes are very sensitive to DNA contamination. PCR master mixes are commonly contaminated with small amounts of DNA which can confound sequencing data, particularly in cases where the sample is low in DNA.
The authors of the paper found that the main contributor to DNA contamination in their workflow was the master mix. By treating the master mix with ArcticZymes’ PCR Decontamination Kit prior to amplification, a 99% reduction in total number of contaminating sequences was observed. The authors conclude that decontamination of PCR reagents in microbiome analysis workflows could have a significant impact on both the accuracy and sensitivity when working with low-biomass samples.
ArcticZymes’ PCR Decontamination Kit contains dsDNase, a double-strand specific endonuclease that can be irreversibly inactivated by a short incubation step at moderate temperatures. Implementation of the PCR Decontamination protocol in sequencing workflows is easy, fast, and does not compromise the integrity of master mix-components such as polymerases or primers.
The PCR Decontamination is available in the webshop.
Stinson, L. , Keelan, J. and Payne, M. (2018), Identification and removal of contaminating microbial DNA from PCR reagents: impact on low‐biomass microbiome analyses. Lett Appl Microbiol. doi:10.1111/lam.13091
The team of Anders Ståhlberg (Sahlgrenska Cancer Center at University of Gothenburg) in collaboration with ArcticZymes has today published an article in International Journal of Molecular Sciences.
The article, titled “Preamplification with dUTP and Cod UNG Enables Elimination of Contaminating Amplicons”, describes how the use of dUTPs in combination with Cod UNG in targeted preamplification can provide a simple and efficient solution for eliminating carry-over contamination.
Carry-over contamination can pose significant challenges when analysing small samples of DNA and RNA where preamplification is required. By introducing contamination clean-up with Cod UNG prior to preamplification with dUTP in the work-flow, these challenges can be minimized.