In a PLoS One article the paleaogenome group of the Institut Jacques Monod, France, Sophie Champlot Camille Berthelot, Mélanie Pruvost, E. Andrew Bennett, Thierry Grange, and Eva-Maria Geigl have been discussing Cod UNG, dsDNase, and HL-dsDNase.
PCR amplification of minute quantities of degraded DNA for ancient DNA research, forensic analyses, wildlife studies and ultrasensitive diagnostics is often hampered by contamination problems. The extent of these problems is inversely related to DNA concentration and target fragment size and concern (i) sample contamination, (ii) laboratory surface contamination, (iii) carry-over contamination, and (iv) contamination of reagents.
Here we performed a quantitative evaluation of current decontamination methods for these last three sources of contamination, and developed a new procedure to eliminate contaminating DNA contained in PCR reagents. We observed that most current decontamination methods are either not efficient enough to degrade short contaminating DNA molecules, rendered inefficient by the reagents themselves, or interfere with the PCR when used at doses high enough to eliminate these molecules. We also show that efficient reagent decontamination can be achieved by using a combination of treatments adapted to different reagent categories. Our procedure involves γ- and UV-irradiation and treatment with a mutant recombinant heat-labile double-strand specific DNase from the Antarctic shrimp Pandalus borealis. Optimal performance of these treatments is achieved in narrow experimental conditions that have been precisely analyzed and defined herein.
There is not a single decontamination method valid for all possible contamination sources occurring in PCR reagents and in the molecular biology laboratory and most common decontamination methods are not efficient enough to decontaminate short DNA fragments of low concentration. We developed a versatile multistrategy decontamination procedure for PCR reagents. We demonstrate that this procedure allows efficient reagent decontamination while preserving the efficiency of PCR amplification of minute quantities of DNA.
On September 29th a delegation of Norwegian ministers are visiting Tromsø to learn about the Bioprospecting activities in the area, in alignment with the governments announced plans to strengthen the activities as an investment into the future of the North.
The delegation is led by the Norwegian Prime Minister Jens Stoltenberg, but also the Minister of Local Government and Regional Development, Liv-Signe Navarsete, the Minister of Fisheries and Coastal Affairs, Lisbeth Berg-Hansen and the Minister of Education, Kristin Halvorsen will be present.
Next to Marbank represented by Manager Kjersti Lie Gabrielsen and MabCent and other bioprospecting activities represented by Prof. Trond Jørgensen, Jan Buch Andersen will represent Marine Biochemicals.
During a short boat trip on the M/S Polargirl each party will present their work and perspectives for development to give the ministers further insights into the regional perspectives of the Bioprospecting activities.
Dun & Bradstreet have recently launched their evaluation of the credit status of Marine Biochemicals and have rated us AA based on the financial result 2009 reported for Marine Biochemicals since its establishment on 3 June 2009 until year end 2009.
Let us just for the record recap the annual result for the full year including the first half year under Biotec Pharmacon: Total revenues: 17.6 mNOK and EBITDA: 8,4 mNOK.