Developed at ArcticZymes, first launched in 1993. Originates from the Arctic Shrimp (Pandalus borealis), from 2010 it has been produced as a recombinant version (rSAP). rSAP is better suited than native SAP (nSAP) for newer, more sensitive technologies.
Shrimp Alkaline Phosphatase (rSAP) has become one of today’s most-selling DNA modifying enzymes due to the added convenience through a complete heat inactivation. While most other alkaline phosphatases (from E. coli or Calf intestine) must be removed by extraction procedures, rSAP is completely inactivated after 15 minutes at 65°C. rSAP works well in common buffers without the requirement for other additions.
Main advantages with rSAP
- Very high specific activity
- 100% heat-inactivated at 65°C
- Removes 5’-phosphates from DNA, RNA, dNTPs and proteins
- May be added directly to restriction enzyme digests
- No vector purification necessary
- Requires no supplemental zinc or other additives for activity
- Works directly in many different buffers
- Easy treatment of unincorporated dNTPs in PCR products prior to DNA sequencing or SNP analysis