ArcticZymes Proteinase
ArcticZymes Proteinase is an unspecific endopeptidase originating from an Arctic marine microbial source. It has broad substrate specificity and is easy to inactivate after use.
Histones and other proteins are known to protect nucleic acids from interacting optimally with other DNA binding proteins and enzymes. ArcticZymes Proteinase is ideally suited for transforming chromatin and other dense nucleic acids to naked DNA. The enzyme is easy to heat-inactivate. This allows thermal inactivation at temperatures allowing RNA integrity as well as avoiding dissociation of dsDNA.
Easy to inactivate
Active at high salt
Compatible with downstream analyses
Glycerol FREE option
Figure 1. ArcticZymes Proteinase is easy to inactivate after use. Figure shows remaining activity after incubation of ArcticZymes Proteinase at different temperatures. Incubation for 30 minutes at indicated temperature in presence of 50 (solid line) or 300 (dotted line) mM NaCl. Remaining activity in % of sample kept on ice in same buffer.
Properties
- Source: Recombinantly produced in Pichia pastoris
Specific activity: >30 Units/mg - Size: 34 kDa
- Unit definition: One unit is defined as the amount of enzyme that produces one µmol 4-nitroaniline (extinction coefficient 8.8 mM-1 at 410 nm) at 25°C, pH 8.0 per minute (buffer conditions 50 mM Tris-HCl pH 8.0, 1% DMSO, 1 mM Suc-Ala-Ala-Pro-Phe-pNA).
- Specificity: Nonspecific endopeptidase
- Activity:
- Highly active in temperature range of 25–40°C
- pH: 7–10
- Tolerance to typical buffer additives:
- SDS: 0.2–1%
- Urea: 1–5 mM
- Inhibitors: The enzyme is inhibited by general serine protease inhibitors, such as PMSF.
- Storage: The enzyme is stable upon storage at -20°C for up to 2 years. ArcticZymes Proteinase FREE is stable upon storage at -20°C for up to 1 year. Additional stability data is available on request.
- Storage buffer:
- 25 mM Tris-HCl, pH 8.0 (25°C)
- 100 mM NaCl
- 50% Glycerol
- Storage buffer (Glycerol FREE):
- 25 mM Tris-HCl, pH 8.0 (25°C)
- 100 mM NaCl
- 1 M Sucrose
ArcticZymes Proteinase Glycerol FREE – lyophilization and automation friendly
Quality Control
dsDNA endonuclease activity
ArcticZymes Proteinase is tested in a reaction containing a supercoiled plasmid for 4 hours at 37°C. No conversion of closed circular DNA to nicked DNA determined by no visible change in band pattern from control sample by agarose gel electrophoresis.
ssDNA endonuclease activity
ArcticZymes Proteinase is tested in a reaction containing M13 ssDNA for 4 hours at 37°C. No conversion of closed circular DNA to open linearized DNA determined by no visible change in band pattern from control sample by agarose gel electrophoresis.
RNase
activity
ArcticZymes Proteinase is tested in a reaction containing a RNA transcript for 4 hours at 37°C. No RNase activity determined by no visible change in band pattern from control sample by agarose gel electrophoresis.
Additional Data and Information
We are pleased to provide additional data and information relating to ArcticZymes Proteinase upon request. We appreciate suggestions for product modifications.
Disclaimer: ArcticZymes Technologies ASA’s, including its subsidiaries, (“ArcticZymes”) products are intended for the further use of in manufacturing new products or research only. Certain applications of ArcticZymes’ products may require licenses from third parties. It is the express duty of any receiver of our products to acquire such licenses, if necessary. To the maximum extent allowed by law, ArcticZymes will not be accountable or liable for any damages, whether direct, indirect, incidental, or consequential, in connection with or arising from this document, including the use of it. ArcticZymes products may be covered by pending or issued patents, designs or design applications and/or trademarks or trademark applications or any other registered or unregistered Intellectual Property Right. For the avoidance of doubt, the General Conditions of ArcticZymes shall apply with respect to any and all purchases and use of the ArcticZymes products.