ArcticZymes Proteinase is an unspecific endopeptidase originating from an Arctic marine microbial source. It has broad substrate specificity and is easy to inactivate after use.
Histones and other proteins are known to protect nucleic acids from interacting optimally with other DNA binding proteins and enzymes. ArcticZymes Proteinase is ideally suited for transforming chromatin and other dense nucleic acids to naked DNA. The enzyme is easy to heat-inactivate. This allows thermal inactivation at temperatures allowing RNA integrity as well as avoiding dissociation of dsDNA.
Easy to inactivate
Active at high salt
Compatible with downstream analyses
Figure 1. ArcticZymes Proteinase is easy to inactivate after use. Figure shows remaining activity after incubation of ArcticZymes Proteinase at different temperatures. Incubation for 30 minutes at indicated temperature in presence of 50 (solid line) or 300 (dotted line) mM NaCl. Remaining activity in % of sample kept on ice in same buffer.
- Source: Recombinantly produced in Pichia pastoris
Specific activity: >30 Units/mg
- Size: 34 kDa
- Unit definition: One unit is defined as the amount of enzyme that produces one µmol 4-nitroaniline (extinction coefficient 8.8 mM-1 at 410 nm) at 25°C, pH 8.0 per minute (buffer conditions 50 mM Tris-HCl pH 8.0, 1% DMSO, 1 mM Suc-Ala-Ala-Pro-Phe-pNA).
- Specificity: Nonspecific endopeptidase
- Highly active in temperature range of 25–40°C
- pH: 7–10
- Tolerance to typical buffer additives:
- SDS: 0.2–1%
- Urea: 1–5 mM
- Inhibitors: The enzyme is inhibited by general serine protease inhibitors, such as PMSF.
- Storage: The enzyme is stable upon storage at -20°C for >1 year. ArcticZymes Proteinase is also stable upon storage at 4°C (>6 months) and room temperature (>4 weeks). ArcticZymes Proteinase is stable at dilute concentrations (<0.1 mg/ml) in solutions.
- Storage buffer:
- 25 mM Tris-HCl, pH 8.0
- 100 mM NaCl
- 50% glycerol
dsDNA endonuclease activity
ArcticZymes Proteinase is tested in a reaction containing a supercoiled plasmid for 4 hours at 37°C. No conversion of closed circular DNA to nicked DNA determined by no visible change in band pattern from control sample by agarose gel electrophoresis.
ssDNA endonuclease activity
ArcticZymes Proteinase is tested in a reaction containing M13 ssDNA for 4 hours at 37°C. No conversion of closed circular DNA to open linearized DNA determined by no visible change in band pattern from control sample by agarose gel electrophoresis.
ArcticZymes Proteinase is tested in a reaction containing a RNA transcript for 4 hours at 37°C. No RNase activity determined by no visible change in band pattern from control sample by agarose gel electrophoresis.
Additional Data and Information
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