Enzymes that Unlock
Your Next Discovery
0
AZscriptᵀᴹ Reverse Transcriptase

AZscriptᵀᴹ Reverse Transcriptase

OverView

AZscript™ Reverse Transcriptase (RT) is an RNA-dependent DNA polymerase used to synthesize complementary DNA (cDNA) from an RNA template.

While originating from Moloney Murine Leukemia Virus (M-MLV), AZscript RT has been engineered for increased thermostability and reduced RNase H activity.

In cDNA synthesis a higher reaction temperature can reduce RNA secondary structures and improve efficiency and specificity of the reaction.

Furthermore, a reduction in RNase H activity increases performance of the cDNA synthesis, especially for longer transcripts.

The combination of these tailored features ensures that AZscript Reverse Transcriptase provides an effective and reliable solution for various cDNA synthesis applications.

Key Features 

  • Increased thermostability.
  • Reduced RNase H activity.

Applications

  • cDNA synthesis
  • Demonstrated performance in RT-qPCR

Figures

AZscript Reverse Transcriptase - AZscript shows excellent performance and sensitivity in RT-qPCR
Figure 1. AZscript shows excellent performance and sensitivity in RT-qPCR
AZscript Reverse Transcriptase - AZscript shows excellent performance and sensitivity in RT-qPCR

A serial dilution of MS2 Armored RNA (Asuragen, 52000) was heat lysed (75℃, 5min) and used as template for cDNA synthesis using gene-specific primers, according to manufacturer’s instructions. A volume equivalent to 2 μl of the cDNA was amplified in a 25 μl reaction using TaqPath™ qPCR Master Mix, CG. Data showing amplification plots (A) and standard curve (B) for the dilution series corresponding to 100 000 down to 10 copies of RNA, in triplicate measurements.

AZscript Reverse Transcriptase - AZscript shows equal or better performance compared to a commonly used reverse transcriptase
Figure 2. AZscript shows equal or better performance compared to a commonly used reverse transcriptase
AZscript Reverse Transcriptase - AZscript shows equal or better performance compared to a commonly used reverse transcriptase

A serial dilution of MS2 Armored RNA (Asuragen, 52000) was heat lysed (75℃, 5min) and used as template for cDNA synthesis using gene-specific primers, according to manufacturer’s instructions (ArcticZymes and Vendor A). A volume equivalent to 2 μl of the cDNA was amplified in a 20 μl reaction using TaqPath™ qPCR Master Mix, CG. Data showing average quantification cycle (Cq) for the dilution series corresponding to 100 000 down to 10 copies of RNA, in triplicate measurements. Error bars represents the standard deviation (n=3).  *Reverse transcriptase from Vendor A failed to detect 10 copies (Cq set to 40).

Properties

Specifications
Source

Recombinantly produced in E. Coli

Size

79.5 kDa

Unit Definition

One unit is defined as the amount of enzyme needed to incorporate 1 nmol of dTTP into acid-precipitable material in 10 min at 37°C using poly(A)⋅oligo(dT)15 as template-primer. The enzyme is assayed in 37.5 mM Tris-HCl pH 8.3 @ 25°C, 40 mM KCl, 6 mM MgCl2, 0.75 mg/ml Poly(A), 0.03 mM oligo(dT)15, 10 mM DTT, 0.5 mM dTTP, 0.025 mCi/ml 3H-dTTP, 0.1 mg/ml BSA, 0.02% Triton X-100.

Temperature Optimum (>80%)

38-47°C

Inactivation

70°C, 15 minutes

Suggested 5X reaction buffer

250 mM Tris-HCl pH 8.3 (25°C), 375 mM KCl, 15 mM MgCl2

Storage and Stability

The enzyme is stable at -20°C for up to 9 months in the supplied storage buffer. Keep AZscript RT on ice while handling. Additional data on stability is available on request.

Quality Control

ArcticZymes is dedicated to the quality of our products. AZscript RT is manufactured at our ISO 13485 certified facility in Norway.

dsDNA endonuclease activity

200 U AZscript RT was incubated with a supercoiled plasmid (1 µg) for 4 hours at 37°C. Agarose gel electrophoresis did not reveal any transformation of closed circular DNA to nicked DNA.

ssDNA endonuclease activity

200 U AZscript RT was incubated with M13 ssDNA (0.5 µg) for 4 hours at 37°C. Agarose gel electrophoresis did not reveal any visible signs of ssDNA degradation.

ssDNA and dsDNA exonuclease activity

200 U AZscript RT was incubated with either 3H-dATP labelled ds or ssDNA (0.5 µg, 500 bp) for 4 hours at 37°C. Acid soluble radioactivity from labelled DNA was not significantly over blank test for either substrate.

RNase activity

200 U AZscript RT was incubated with a 2 kb RNA transcript (1 µg) for 4 hours at 37°C. Agarose gel electrophoresis did not reveal any visible signs of RNA degradation.

Recommended Protocols

Ordering Info

Quantity
Product Name
SKU
Pack Size
Concentration
Price
1
T7 RNA Polymerase
74100-201
10 000 U
50 U/µl
$ 145.00 USD
133.00
145.00
Request Quote
2
T7 RNA Polymerase
74100-110
100 kU
≥ 500 U/µl
$ 0.00 USD
Request Quote
3
T7 RNA Polymerase
74100-100
As per request
As per request
$ 0.00 USD
Request Quote
4
T7 RNA Polymerase Glycerol Free
74110-110
100 kU
≥ 500 U/µl
$ 0.00 USD
Request Quote
5
T7 RNA Polymerase Glycerol Free
74110-100
As per request
As per request
$ 0.00 USD
Request Quote
1
T4 DNA Ligase
71800-202
250 kU
5000 U/μl
$ 32.00 USD
30.00
32.00
Request Quote
1
AZscript RT
72100-201
10000 U
200 U/μl
$ 136.00 USD
123.00
136.00
Request Quote
1
Heat&Run gDNA Removal Kit
80200-250
250 rxn
$ 651.00 USD
548.00
651.00
Request Quote
1
M-SAN HQ ELISA Kit
70960-001
12x8 Strip plate
$ 756.00 USD
655.00
756.00
Request Quote
1
HL-dsDNase Triton Free
70801-201
250 U
2 U/µl
$ 357.00 USD
305.00
357.00
Request Quote
1
PCR Decontamination Kit
80400-100
100 rxn
$ 130.00 USD
110.00
130.00
Request Quote
1
AZtaq™ DNA Polymerase
73100-201
500 U
5 U/µl
$ 83.00 USD
75.00
83.00
Request Quote
1
HL-SAN Triton Free
70911-202
25000 U
25 U/µl
$ 560.00 USD
473.00
560.00
Request Quote
1
ArcticZymes Proteinase
71600-201
50 U
>200 U/ml
$ 155.00 USD
131.00
155.00
Request Quote
1
IsoPol®
71500-201
200 U
5 U/µl
$ 75.00 USD
68.00
75.00
Request Quote
1
ArcticZymes R2D Ligase
71900-105
5 kU
10 U/µl
$ 419.00 USD
361.00
419.00
Request Quote
1
rSAP
70700-201
1000 U
1 U/µl
$ 131.00 USD
116.00
131.00
Request Quote
1
dsDNase Triton Free
70601-201
250 U
2 U/µl
$ 357.00 USD
305.00
357.00
Request Quote
1
Cod UNG Triton Free
70501-201
100 U
1 U/µl
$ 130.00 USD
109.00
130.00
Request Quote
1
IsoPol™ BST⁺
71502-202
1600 U
8 U/µl
$ 78.00 USD
71.00
78.00
Request Quote
1
SAN High Quality
70920-150
500 kU
>250 U/µl
$ 5,767.00 USD
5011.00
5767.00
Request Quote
1
HL-ExoI
70100-201
2500 U
>20 U/µl
$ 69.00 USD
65.00
69.00
Request Quote
1
SAN HQ 2.0
70940-150
500 kU
250 U/µl
$ 5,767.00 USD
5011.00
5767.00
Request Quote
2
Cod UNG Triton Free
70501-202
1000 U
1 U/µl
$ 548.00 USD
456.00
548.00
Request Quote
2
SAN High Quality
70920-160
5 MU
>250 U/µl
$ 21,913.00 USD
19035.00
21913.00
Request Quote
2
PCR Decontamination Kit
80400-500
500 rxn
$ 519.00 USD
445.00
519.00
Request Quote
2
HL-dsDNase Triton Free
70801-202
1000 U
2 U/µl
$ 1,055.00 USD
877.00
1055.00
Request Quote
2
IsoPol™ BST⁺
71502-120
10000 U
120 U/µl
$ 384.00 USD
350.00
384.00
Request Quote
2
dsDNase Triton Free
70601-202
1000 U
2 U/µl
$ 1,055.00 USD
877.00
1055.00
Request Quote
2
T4 DNA Ligase
71800-100
as per request
as per request
$ 0.00 USD
Request Quote
2
ArcticZymes R2D Ligase
71900-100
As per request
As per request
$ 0.00 USD
Request Quote
2
M-SAN HQ
70950-120
200 kU
>250 U/µl
$ 3,155.00 USD
3024.00
3155.00
Request Quote
2
IsoPol® SD+
71501-201
200 U
5 U/µl
$ 75.00 USD
68.00
75.00
Request Quote
2
HL-SAN Triton Free
70911-150
500 kU
25 U/µl
$ 5,425.00 USD
4714.00
5425.00
Request Quote
2
rSAP
70700-202
5000 U
1 U/µl
$ 519.00 USD
450.00
519.00
Request Quote
2
SAN HQ 2.0
70940-160
5 MU
>250 U/µl
$ 21,913.00 USD
19035.00
21913.00
Request Quote
2
Heat&Run gDNA Removal Kit
80200-50
50 rxn
$ 166.00 USD
139.00
166.00
Request Quote
2
HL-ExoI
70100-202
500 kU
>20 U/µl
$ 6,148.00 USD
5040.00
6148.00
Request Quote
2
AZscript RT
72100-100
As per request
As per request
$ 0.00 USD
Request Quote
2
ArcticZymes Proteinase
71600-110
1000 U
>200 U/ml
$ 1,419.00 USD
1188.00
1419.00
Request Quote
2
AZtaq™ DNA Polymerase
73100-110
5000 U
>50 U/µl
$ 619.00 USD
568.00
619.00
Request Quote
3
Cod UNG Triton Free
70501-110
10 kU
1 U/µl
$ 3,920.00 USD
3472.00
3920.00
Request Quote
3
SAN HQ 2.0
70940-202
25 kU
25 - 30 U/µl
$ 577.00 USD
498.00
577.00
Request Quote
3
rSAP
70700-203
25 kU
1 U/µl
$ 1,965.00 USD
1703.00
1965.00
Request Quote
3
HL-ExoI
70100-150
50 kU
>20 U/µl
$ 683.00 USD
593.00
683.00
Request Quote
3
M-SAN HQ
70950-150
500 kU
>250 U/µl
$ 6,055.00 USD
5803.00
6055.00
Request Quote
3
ArcticZymes Proteinase
71600-100
As per request
>200 U/ml
$ 0.00 USD
Request Quote
3
IsoPol® BST⁺ Glycerol Free
71512-100
As per request
As per request
$ 0.00 USD
Request Quote
3
HL-dsDNase Triton Free
70801-203
2500 U
5 U/µ
$ 2,365.00 USD
1961.00
2365.00
Request Quote
3
AZtaq™ DNA Polymerase
73100-100
As per request
As per request
$ 0.00 USD
Request Quote
3
dsDNase Triton Free
70601-203
2500 U
5 U/µl
$ 2,365.00 USD
1961.00
2365.00
Request Quote
3
SAN High Quality
70920-202
25 kU
25-30 U/µl
$ 577.00 USD
498.00
577.00
Request Quote
3
HL-SAN
70910-202
25000 U
25 U/µl
$ 560.00 USD
473.00
560.00
Request Quote
4
IsoPol® BST⁺ Glycerol HC Free
71522-100
As per request
As per request
$ 0.00 USD
Request Quote
4
Cod UNG Triton Free
70501-151
50 kU
1 U/µl
$ 14,128.00 USD
12281.00
14128.00
Request Quote
4
ArcticZymes Proteinase Glycerol-FREE
71610-100
As per request
>200 U/ml
$ 0.00 USD
Request Quote
4
rSAP
70700-101
200 kU
1 U/µl
$ 13,375.00 USD
11631.00
13375.00
Request Quote
4
dsDNase Triton Free
70601-150
50 kU
50 U/µl
$ 17,069.00 USD
14830.00
17069.00
Request Quote
4
SAN HQ 2.0 ELISA Kit
70970-001
1x96 Well Plate
$ 755.00 USD
655.00
755.00
Request Quote
4
HL-dsDNase Triton Free
70801-110
100 kU
2 U/µl
$ 45,552.00 USD
39608.00
45552.00
Request Quote
4
HL-SAN
70910-150
500 kU
26 U/µl
$ 5,425.00 USD
4714.00
5425.00
Request Quote
4
SAN High Quality GMP
70980-150
500 kU
400-600 U/ul
$ 0.00 USD
Request Quote
5
Cod UNG Triton Free
70501-150
50 kU
50 U/µl
$ 14,128.00 USD
12281.00
14128.00
Request Quote
5
HL-dsDNase Glycerol-Free - Triton Free
70811-50
5000 U
>50 U/µl
$ 4,031.00 USD
3505.00
4031.00
Request Quote
5
rSAP Glycerol-free
70710-201
5000 U
>20 U/µl
$ 603.00 USD
521.00
603.00
Request Quote
4
M-SAN HQ
70950-155
1 MU
>250 U/µl
$ 10,905.00 USD
10450.00
10905.00
Request Quote
5
M-SAN HQ
70950-160
5 MU
>250 U/µl
$ 23,008.00 USD
22049.00
23008.00
Request Quote
5
dsDNase Triton Free
70601-110
100 kU
2 U/µl
$ 25,604.00 USD
22260.00
25604.00
Request Quote
5
SAN High Quality GMP
70980-160
5 MU
400-600 U/µl
$ 0.00 USD
Request Quote
6
rSAP Glycerol-free
70710-100
As per request
As per request
$ 0.00 USD
Request Quote
6
Cod UNG Glycerol-Free Triton Free
70511-105
5000 U
50 U/µl
$ 2,598.00 USD
2256.00
2598.00
Request Quote
6
dsDNase Glycerol Free, Triton Free
70611-50
5000 U
>50 U/µl
$ 4,031.00 USD
3505.00
4031.00
Request Quote
6
SAN High Quality Triton Free
70921-150
500 kU
>250 U/µl
$ 5,767.00 USD
5011.00
5767.00
Request Quote
6
M-SAN HQ
70950-202
25 kU
25-30 U/µl
$ 606.00 USD
580.00
606.00
Request Quote
6
HL-dsDNase
70800-201
250 U
2 U/µl
$ 357.00 USD
305.00
357.00
Request Quote
7
Cod UNG Glycerol-Free Triton Free
70511-150
50 kU
50 U/µl
$ 14,128.00 USD
12281.00
14128.00
Request Quote
7
SAN High Quality Triton Free
70921-160
5 MU
>250 U/µl
$ 21,913.00 USD
19035.00
21913.00
Request Quote
7
dsDNase
70600-201
250
2 U/µl
$ 357.00 USD
305.00
357.00
Request Quote
7
HL-dsDNase
70800-202
1000 U
2 U/µl
$ 1,055.00 USD
877.00
1055.00
Request Quote
8
SAN High Quality Triton Free
70921-202
25 kU
25-30 U/µl
$ 577.00 USD
498.00
577.00
Request Quote
8
Cod UNG
70500-201
100 U
1 U/µl
$ 130.00 USD
109.00
130.00
Request Quote
8
dsDNase
70600-202
1 000
2 U/µl
$ 1,055.00 USD
877.00
1055.00
Request Quote
8
HL-dsDNase
70800-203
2500 U
5 U/µl
$ 2,365.00 USD
1961.00
2365.00
Request Quote
9
Cod UNG
70500-202
1000 U
1 U/µl
$ 548.00 USD
456.00
548.00
Request Quote
9
SAN High Quality ELISA Kit
70930-001
1x96 Well Compact Plate Format
$ 756.00 USD
655.00
756.00
Request Quote
9
dsDNase
70600-203
2 500
5 U/µl
$ 2,365.00 USD
1961.00
2365.00
Request Quote
9
HL-dsDNase
70800-150
50 kU
50 U/µl
$ 30,330.00 USD
26368.00
30330.00
Request Quote
10
dsDNase
70600-150
50 kU
50 U/µl
$ 17,069.00 USD
14830.00
17069.00
Request Quote
10
Cod UNG
70500-110
10 kU
1 U/µl
$ 3,920.00 USD
3472.00
3920.00
Request Quote
10
HL-dsDNase
70800-110
100 kU
2 U/µl
$ 45,552.00 USD
39608.00
45552.00
Request Quote
11
dsDNase
70600-110
100 kU
2 U/µl
$ 25,604.00 USD
22260.00
25604.00
Request Quote
11
Cod UNG
70500-151
50 kU
1 U/µl
$ 14,128.00 USD
12281.00
14128.00
Request Quote
11
HL-dsDNase
70800-120
500 kU
2 U/µl
$ 170,682.00 USD
148412.00
170682.00
Request Quote
12
Cod UNG
70500-150
50 kU
50 U/µl
$ 14,128.00 USD
12281.00
14128.00
Request Quote
12
dsDNase Glycerol Free
70610-50
5000 U
80 - 140 U/µl
$ 4,031.00 USD
3505.00
4031.00
Request Quote
12
HL-dsDNase Glycerol-free
70810-50
5000 U
80 - 140 U/µl
$ 4,031.00 USD
3505.00
4031.00
Request Quote
13
Cod UNG Glycerol Free
70510-105
5000 U
50 U/µl
$ 2,598.00 USD
2256.00
2598.00
Request Quote
14
Cod UNG Glycerol Free
70510-150
50 kU
50 U/µl
$ 14,128.00 USD
12281.00
14128.00
Request Quote
Note: Article no. 71502-202 and 71502-120 are provided with IsoPol® BST+ 10X Buffer and MgSO4 .
Add To Cart
Right arrow.

Publications

We are using a plug-in called Bioz.com who will provide a script to add to Webflow. This will pull in all the publications.

We don’t believe this will impact the overall layout but it may offer some opportunities for example adding a «Citations» button next to the ordering info. We’ll find out later in August as form the relationship

No items found.

FAQ's

No items found.
For Customer Support
For  Technical Support
No items found.
AZscriptᵀᴹ Reverse Transcriptase
SAN HQ
now available in
GMP grade