Salt Active Nuclease
High Quality (Bioprocessing grade)

SAN High Quality (Bioprocessing grade) is the ultimate solution for efficient removal of nucleic acids in manufacturing and bioprocessing workflows. This nonspecific, recombinant endonuclease has optimum activity at high salt concentrations, which can improve efficiency and yield in various workflows.

Salt is an important component of various purification schemes. The presence of salt can minimize aggregation, increase target solubility and improve target yield. High salt enables contaminating DNA to dissociate from associated proteins and become available for degradation. SAN High Quality is highly compatible with the use of high salt conditions.


High activity at high salt conditions


Supplied with extended product documentation


Compatible with SAN HQ ELISA


High purity (≥ 98%)


No protease detected


Active at low temperatures


Figure 1. SAN High Quality outperforms other endonucleases in solutions with high salt. Comparison of activity using two commercially available endonucleases and SAN High Quality. The endonucleases were tested at different concentrations of NaCl (0M, 0.25M and 0.5M) and temperatures (6°C, 25°C, 37°C).


Figure 2. Optimum activity in solutions with high salinity. SAN High Quality has optimum activity at 0.5M NaCl, but operates at a broad range of NaCl and KCl. The activity was tested in a 25mM Tris-HCl buffer, pH 8.5, 5mM MgCl2 with varying concentrations of NaCl and KCl. Maximum activity was set to 100%


Figure 3. Buffer compatibility of SAN High Quality. Relative activity of SAN High Quality in presence of various common buffer components. Hundred percent activity was set in standard assay conditions (25 mM Tris-HCl, pH 8.5, 5 mM MgCl2, 0.5 M NaCl).


  • Source: Recombinantly produced in Pichia pastoris
  • Molecular weight: The protein is glycosylated. Protein size without glycosylation is 26 kDa.
  • Protein purity: > 98% by SDS-PAGE analysis
  • Isoelectric point: 9.55
  • Unit definition: One unit is defined as the amount of enzyme that causes a ΔA260 = 1.0 in 30 minutes at 37°C in 25 mM Tris-HCI  pH 8.5 (@25°C), 5 mM MgCl2, 500 mM NaCl, and 50 µg/ml calf thymus DNA.
  • Specificity: Nonspecific endonuclease cleaving single and double stranded DNA and RNA. 
  • Working ranges:
    • Temperature: 5 – 40°C, optimal: ~35°C
    • Salt concentrations (NaCl / KCl): 150 mM – 900 mM, optimal: 400 - 650 mM
    • Mg2+: >1 mM is required for activity, optimal: 5 - 50 mM
    • pH: 7.3 – 10.0, optimal: 8.2 - 9.2                                                                                                                                        

      Note: The working range is defined as ~20% of activity and optimal range is ~80% of activity.

  • Tolerance to typical buffer additives:
    • Imidazole: 20% activity at 350 mM Imidazole
    • Glycerol: 20% activity at 35% glycerol
    • Triton X-100: No reduction in activity (tested up to 15%)
    • SDS: Not recommended
    • Urea: Not recommended
    • Reducing agents (e.g. DTT, TCEP): will result in inactivation


ArcticZymes offers SAN HQ ELISA Kit to confirm the removal of SAN High Quality (Bioprocessing Grade) in bioprocessing and biomanufacturing applications.


SAN-HQ Applications

  1. Moving from the bench towards a large scale, industrial platform process for adeno-associated viral vector purification.
    Adams B, Bak H, Tustian AD.
    Biotechnology & Bioengineering. 2020; 117 (10): 3199-3211.

  2. Cytosine and adenine base editing of the brain, liver, retina, heart and skeletal muscle of mice via adeno-associated viruses.
    Levy JM, Yeh WH, Pendse N, Davis JR, Hennessey E, Butcher R, Koblan LW, Comander J, Liu Q, Liu DR.
    Nature Biomedical Engineering. 2020; 4(1): 97-110.

  3. Global Representations of Goal-Directed Behavior in Distinct Cell Types of Mouse Neocortex.
    Allen WE, Kauvar IV, Chen MZ, Richman EB, Yang SJ, Chan K, Gradinaru V, Deverman BE, Luo L, Deisseroth.
    Neuron. 2017; 94 (4): 891-907.

  4. Identification of peripheral neural circuits that regulate heart rate using optogenetic and viral vector strategies.
    Rajendran PS, Challis RC, Fowlkes CC, Hanna P, Tompkins JD, Jordan MC, Hiyari S, Gabris-Weber BA, Greenbaum A, Chan KY, Deverman BE, Münzberg H, Ardell JL, Salama G, Gradinaru V, Shivkumar K.
    Nat Commun. 2019; 10: 1944.

  5. Multiplexed peroxidase-based electron microscopy labeling enables simultaneous visualization of multiple cell types.
    Zhang Q, Lee WA, Paul DL, Ginty DD.
    Nat Neurosci. 2019; 22: 828–839.

  6. Near physiological spectral selectivity of cochlear optogenetics.
    Dieter A, Duque-Afonso CJ, Rankovic V, Jeschke M, Moser T.
    Nat Commun. 2019; 10: 1962.

  7. Mining, analyzing, and integrating viral signals from metagenomic data.
    Zheng T, Li J, Ni Y, Kang K, Misiakou MA, Imamovic L, Chow BKC, Rode AA, Bytzer P, Sommer M, Panagiotou G.
    Microbiome. 2019; 7: 42.

  8. Structures of the Human PGD2 Receptor CRTH2 Reveal Novel Mechanisms for Ligand Recognition.
    Wang L, Yao D, Krishna Deepak RNV, Liu H, Xiao Q, Fan H, Gong W, Wei Z, Zhang C.
    Molecular Cell. 2018; 72, 48-59.

  9. Ultrafast optogenetic stimulation of the auditory pathway by targeting-optimized Chronos. 
    Keppeler D, Merino RM, Lopez de la Morena D, Bali B, Huet AT, Gehrt A, Wrobel C, Subramanian S, Dombrowski T, Wolf F, Rankovic V, Neef A, Moser T.
    EMBO J. 2018; 37(24): e99649.

  10. Engineered AAVs for efficient noninvasive gene delivery to the central and peripheral nervous systems. 
    Chan K, Jang M, Yoo B, Greenbaum A, Ravi N, Wu WL, Sánchez-Guardado L, Mazmanian SK, Deverman BE, Gradinaru V.
    Nat Neurosci. 2017; 20: 1172–1179.

  11. Global Representations of Goal-Directed Behavior in Distinct Cell Types of Mouse Neocortex.
    Allen WE, Kauvar IV, Chen MZ, Richman EB, Yang SJ, Chan K, Gradinaru V, Deverman BE, Luo L, Deisseroth K.
    Neuron. 2017; 94 (4): 891-907.e6.