Heat&Run Genomic DNA removal kit FAQ

  1. Is H&R compatible with RNase inhibitor?
  2. Is H&R compatible with RNAlater (Life) and similar reagents?
  3. Can RNA treated with H&R be stored?
  4. Can H&R be used together with any reverse transcription kit?
  5. What sources of RNA can be used for H&R cleanup?
  6. Can RNA dissolved in TE buffer be used for H&R cleanup?
  7. What kind of RNA isolation is recommended for RNA being treated with H&R?

Is H&R compatible with RNase inhibitor?

Yes – RNase inhibitor does not affect the performance of the H&R kit.


Is H&R compatible with RNAlater (Life) and similar reagents?

Not tested


Can RNA treated with H&R be stored?

Yes – but be aware that Mg2+ remains in the solution after H&R treatment, which might reduce the long-term stability of RNA. If RNA storage after treatment is desired, add EDTA to a final concentration of 3mM after H&R treatment.


Can H&R be used together with any reverse transcription kit?

Yes – However some Mg2+ remains in the RNA after H&R clean-up. In some cases this can interfere with RT performance. If this is the case, simply add EDTA to a final concentration of 3 mM prior to RT.


What sources of RNA can be used for H&R cleanup?

All sources of RNA are compatible with H&R. Successful gDNA removal has been shown in RNA preps from several bacterial, animal and plant species.


Can RNA dissolved in TE buffer be used for H&R cleanup?

No – H&R is dependent on Mg2+ to function, thus TE buffer will reduce the activity of the kit.


What kind of RNA isolation is recommended for RNA being treated with H&R?

Any RNA isolation method is suitable. However, avoid elution of / dissolving RNA using TE buffer.