The kit contains HL-dsDNase which efficiently removes gDNA from RNA preps.
- gDNA is removed, leaving RNA ready for reverse transcription in the same tube (Figure 1).
- Heat-labile dsDNase can easily be inactivated without reducing the quality or quantity of RNA.
- This procedure minimizes pipetting steps and reduces hands-on time.
- The Heat&Run kit is especially well suited for high throughput experiments.
The high activity of the HL-dsDNase at low temperature and its heat-lability makes it ideal to use in a combination with a heat activated RT enzyme. The contaminating genomic DNA is cleave at ambient temperature and increasing the temperature over 50°C will inactivate the DNase and start the Reverse Transcriptase.
Heat&Run causes no harmful effect on the amount of RNA being detected in subsequent RT-qPCR. In our experiments, neither mRNA, miRNA nor lnc-RNA was lost or damaged, while gDNA was efficiently and completely removed.
|Figure 2: qPCR shows the Heat&Run kit removes at least 50ng of gDNA in a 10µl reaction volume.|
- 10X reaction Buffer
- HL-dsDNase (50 reactions)
Storage Conditions: Store at -20˚C.
Sample Material: The Heat&Run kit is suited for gDNA removal of RNA from any source.
Quality Control: The kit is tested for absence of RNase.
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Received 14 May 2013 Accepted 01 July 2014 Published online 24 August 2014