M-SAN High Quality nuclease

The main advantages of M-SAN High Quality

  • High purity (≥ 98%)
  • No protease detected
  • High activity at physological salt conditions
  • Supplied with extended product documentation

Overview

M-SAN HQ is the latest addition to our high salt tolerance nucleases portfolio. The biochemical properties of the SAN family of nucleases make them ideal for multiple bioprocessing and biomanufacturing workflows.

M-SAN HQ has been developed to offer the optimal solution for removal of nucleic acids at the near-physiological conditions used in many bioprocessing and biomanufacturing workflows. This novel, nonspecific endonuclease is active over a broad pH range and displays optimum activity at salt concentrations around 175 mM.

 

 

 

 

 

Figure 1
M-SAN HQ has high activity in the 125 – 250
mM salt range. Activity was measured at 37°C in 25 mM
Tris-HCl pH 7.4 @ 37°C, 2.5 mM MgCl2, 50 µg/ml calf
thymus DNA.

Figure 2
M-SAN HQ has high activity over a broad
pH range. Activity (ΔAbs260) was measured at 37°C in
25 mM Tris-HCl (blue) or 25 mM Bis-Tris (gray), 150
mM NaCl, 2.5 mM MgCl2, 50 µg/ml calf thymus DNA.

 

 

 

 

 

 

The majority of commercially available nucleases display limited performance at salt concentrations and pHs typically used in cell cultures. This can now be overcome by using ArcticZymes’ M-SAN HQ where these conditions are close to optimal range, reducing additional steps in the workflows and thus increase efficiency and reduce costs.

 

 

 

 

 

 

Figure 3
Activity of M-SAN HQ relative to a competitor
nuclease. Equal amounts (as per manufacturers unit
definition) of M-SAN HQ and Nuclease B was tested at
various NaCl concentrations in 25 mM Tris-HCl pH 7.2 @
37°C, 5 mM MgCl2, 50 µg/ml calf thymus DNA. Values are
expressed as relative activities compared to M-SAN HQ

Figure 4
Activity of M-SAN HQ compared to a
competitor nuclease. Activity was measured in 25 mM
Tris-HCl pH 7.2 @ 37°C, 5 mM MgCl2, 50 µg/ml calf
thymus DNA, expressed as ΔAbs260/min.

 

Properties

  • Source: Recombinantly produced in Pichia pastoris
  • Size: Protein size is 24.5 kDa.
  • pI: 8.62.
  • Unit definition: One Unit is defined as an increase in absorbance at 260 nm of 1 A in 30 minutes at 37°C, using 50 μg/ml calf thymus DNA in a buffer consisting of 25 mM Tris-HCl pH 7.6 @ 25°C, 2.5 mM MgCl2, 150 mM NaCl.
  • Activity:
    • Tmp: 25–40°C, optimal 37°C
    • NaCl: Working range (>20%): 50-400mM. Optimal range (>80%): 125mM–250mM
    • Mg2+ (>1mM) is required for activity, optimal range Mg2+ is >4 mM
    • pH: Working range (>20%): 6.5-10.0. Optimal range (>80%): 7.2–9.5
  • Tolerance to typical buffer additives:
    • DTT and other reducing agents may inactivate M-SAN HQ
    • Urea: Not recommended
    • EDTA: Not recommended