ArcticZymes Proteinase

Main advantages

  • Easy to inactivate
  • Active at high salt
  • Compatible with downstream analyses

Overview

ArcticZymes Proteinase is an unspecific endopeptidase originating from an Arctic marine microbial source. It has broad substrate specificity and is easy to inactivate after use.

Histones and other proteins are known to protect nucleic acids from interacting optimally with other DNA binding proteins and enzymes. ArcticZymes Proteinase is ideally suited for transforming chromatin and other dense nucleic acids to naked DNA. The enzyme is easy to heat-inactivate. This allows thermal inactivation at temperatures allowing RNA integrity as well as avoiding dissociation of dsDNA.

Figure 1. ArcticZymes Proteinase is easy to inactivate after use. Figure shows remaining activity after incubation of ArcticZymes Proteinase at different temperatures. Incubation for 30 minutes at indicated temperature in presence of 50 (solid line) or 300 (dotted line) mM NaCl. Remaining activity in % of sample kept on ice in same buffer.

Properties

  • Source: Recombinantly produced in Pichia pastoris
  • Specific activity: >30 Units/mg
  • Size: 34 kDa
  • Unit definition: One unit is defined as the amount of enzyme that produces one µmol 4-nitroaniline (extinction coefficient 8.8 mM-1 at 410 nm) at 25°C, pH 8.0 per minute (buffer conditions 50 mM Tris-HCl pH 8.0, 1% DMSO, 1 mM Suc-Ala-Ala-Pro-Phe-pNA).
  • Specificity: Nonspecific endopeptidase
  • Activity:
    • Highly active in temperature range of 25–40°C
    • pH: 7–10
  • Tolerance to typical buffer additives:
    • SDS: 0.2–1%
    • Urea: 1–5 mM
  • Inhibitors: The enzyme is inhibited by general serine protease inhibitors, such as PMSF.
  • Storage: The enzyme is stable upon storage at -20°C for >1 year. ArcticZymes Proteinase is also stable upon storage at 4°C (>6 months) and room temperature (>4 weeks). ArcticZymes Proteinase is stable at dilute concentrations (<0.1 mg/ml) in solutions.
  • Storage buffer:
    • 25 mM Tris-HCl, pH 8.0
    • 100 mM NaCl
    • 50% glycerol

Quality Control

ArcticZymes is dedicated to the quality of our products. We manufacture all our products at our ISO:13485 certified facility in Norway.

dsDNA endonuclease activity

ArcticZymes Proteinase is tested in a reaction containing a supercoiled plasmid for 4 hours at 37°C. No conversion of closed circular DNA to nicked DNA determined by no visible change in band pattern from control sample by agarose gel electrophoresis.

ssDNA endonuclease activity

ArcticZymes Proteinase is tested in a reaction containing M13 ssDNA for 4 hours at 37°C. No conversion of closed circular DNA to open linearized DNA determined by no visible change in band pattern from control sample by agarose gel electrophoresis.

RNase activity

ArcticZymes Proteinase is tested in a reaction containing a RNA transcript for 4 hours at 37°C. No RNase activity determined by no visible change in band pattern from control sample by agarose gel electrophoresis.

Additional Data and Information

We are pleased to provide data and information relating to ArcticZymes Proteinase. The Product is an Early Access Product, and further product information will be generated continuously. We appreciate suggestions for product modifications.

Disclaimer: These products are intended for further manufacturing use or research use only. Certain applications of ArcticZymes AS products may require licenses from others. It is the expressed duty of any receiver of ArcticZymes AS products to acquire such licenses, if necessary. To the extent allowed by law, ArcticZymes AS will not be liable for damages, whether direct, indirect, incidental, or consequential in connection with or arising from this document, including the use of it. ArcticZymes AS products may be covered by pending or issued patents, designs or design applications and/or trademarks or trademark applications or any other registered or unregistered Intellectual Property Right.