Salt Active Nuclease
High Quality (Bioprocessing grade)

The main advantages of SAN High Quality

  • High purity (≥ 98%)
  • No protease detected
  • Active at low temperatures
  • High activity at high salt conditions
  • Supplied with extended product documentation
  • Compatible with SAN HQ ELISA

Overview

SAN High Quality (Bioprocessing grade) is the ultimate solution for efficient removal of nucleic acids in manufacturing and bioprocessing workflows. This nonspecific, recombinant endonuclease has optimum activity at high salt concentrations, which can improve efficiency and yield in various workflows.

Salt is an important component of various purification schemes. The presence of salt can minimize aggregation, increase target solubility and improve target yield. High salt enables contaminating DNA to dissociate from associated proteins and become available for degradation. SAN High Quality is highly compatible with the use of high salt conditions.

Figure 1. SAN High Quality outperforms other endonucleases in solutions with high salt. Comparison of activity using two commercially
available endonucleases and SAN High Quality. The endonucleases were tested at different concentrations of NaCl (0M, 0.25M and 0.5M) and temperatures (6°C, 25°C, 37°C).
Figure 2. Optimum activity in solutions with high salinity. SAN High Quality has optimum activity at 0.5M NaCl, but operates at a broad range of NaCl and KCl. The activity was tested in a 25mM Tris-HCl buffer, pH 8.5, 5mM MgCl2 with varying concentrations of NaCl and KCl. Maximum activity was set to 100%
Figure 3. Buffer compatibility of SAN High Quality. Relative activity of SAN High Quality in presence of various common buffer components. Hundred percent activity was set in standard assay conditions (25 mM Tris-HCl, pH 8.5, 5 mM MgCl2, 0.5 M NaCl).

Properties

  • Source: Recombinantly produced in Pichia pastoris
  • Specific activity: ≥ 175 000 Units/mg
  • Size: The protein is glycosylated. Protein size without glycosylation is 26 kDa
  • Unit definition: One Unit is defined as an increase in absorbance at 260 nm of 1 A in 30 minutes at 37°C, using 50 μg/ml calf thymus DNA in a buffer consisting of 25 mM Tris-HCl, pH 8.5 (25°C), 5 mM MgCl2, 500 mM NaCl
  • Specificity: Nonspecific endonuclease cleaving single- and double stranded DNA and RNA. The enzyme degrades DNA vs. RNA in a 10:1 ratio
  • End-product: Following complete degradation, the end product consist of a majority of 5 nucleotide oligos
  • Activity:
    • Tmp: 0–40°C, optimal 35°C
    • NaCl: 50mM–1M, optimal NaCl is 0.5 M
    • Mg2+ (>1 mM) is required for activity, optimal Mg2+ is 20 mM
    • pH: 7.0–9.5, optimal pH is 9
  • Tolerance to typical buffer additives:
    • Imidazole: 20% activity at 350 mM Imidazole
    • Glycerol: 20% activity at 35% glycerol
    • Triton X-100: No reduction in activity (tested up to 15%)
    • SDS: Not recommended
    • Urea: Not recommended
    • Reducing agents (e.g. DTT, TCEP): will result in inactivation

SAN HQ ELISA Kit

ArcticZymes presents SAN HQ ELISA Kit to confirm the removal of SAN High Quality (Bioprocessing Grade) in nucleic acid manufacturing.  The product completes the ArcticZymes product portfolio in this new and exciting market segment. The SAN HQ ELISA Kit is available for early access purchase.  ArcticZymes will accept purchase orders beginning October 1, 2017 and ship products to early access customers prior to the end of October 2017. The early access price of the SAN HQ ELISA Kit is $649.00 USD through the end of 2017.

Publications

  1. Structural basis of Zika virus methyltransferase inhibition by sinefungin
    Kamil Hercik, Jiri Brynda, Radim Nencka, Evzen Boura
    Archives of Virology, July 2017, Volume 162, Issue 7, pp 2091–2096
  2. Engineered AAVs for efficient noninvasive gene delivery to the central and peripheral nervous systems
    Ken Y Chan, Min J Jang, Bryan B Yoo, Alon Greenbaum, Namita Ravi, Wei-Li Wu, Luis Sánchez-Guardado, Carlos Lois, Sarkis K Mazmanian, Benjamin E Deverman & Viviana Gradinaru
  3. Global Representations of Goal-Directed Behavior in Distinct Cell Types of Mouse Neocortex
    William E. Allen, Isaac V. Kauvar, Michael Z. Chen, Ethan B. Richman, Samuel J. Yang, Ken Chan, Viviana Gradinaru, Benjamin E. Deverman, Liqun Luo
  4. LitR Is a Repressor of syp Genes and Has a Temperature-Sensitive Regulatory Effect on Biofilm Formation and Colony Morphology in Vibrio(Aliivibrio) salmonicida
    Appl. Environ. Microbiol. September 2014 vol. 80 no. 17 5530-5541
    Published ahead of print 27 June 2014, doi: 10.1128/AEM.01239-14