Complete Removal of DNA
Application Overview
Complete removal of DNA from cell lysates is crucial for various molecular biology applications. The complexity of cell lysates, which can contain a mix of DNA, RNA, proteins, lipids, and detergents, often complicates this process. SAN enzymes offer a tailored solution for efficient DNA degradation, adaptable to various lysate compositions.
Application Overview
Complete removal of DNA from cell lysates is crucial for various molecular biology applications. The complexity of cell lysates, which can contain a mix of DNA, RNA, proteins, lipids, and detergents, often complicates this process. SAN enzymes offer a tailored solution for efficient DNA degradation, adaptable to various lysate compositions.
THE PROBLEM These ENZYMEs SOLVE
Cell lysates are complex mixtures that often require specialized treatment for complete DNA removal. Inadequate DNA removal can interfere with downstream applications and analyses. Traditional methods may not be effective or require complicated protocols to ensure complete DNA degradation.
THE PROBLEM These ENZYME SOLVES
Cell lysates are complex mixtures that often require specialized treatment for complete DNA removal. Inadequate DNA removal can interfere with downstream applications and analyses. Traditional methods may not be effective or require complicated protocols to ensure complete DNA degradation.
THE PROBLEM These ENZYMes SOLVE
Cell lysates are complex mixtures that often require specialized treatment for complete DNA removal. Inadequate DNA removal can interfere with downstream applications and analyses. Traditional methods may not be effective or require complicated protocols to ensure complete DNA degradation.
The Solution
SAN enzymes provide a flexible and efficient approach to DNA removal. The amount of SAN needed can be adjusted based on the complexity of the lysate. For optimal results, adjusting the NaCl concentration to 0.5 M and incubating at 4°C overnight can enhance DNA dissociation from proteins. Subsequent SAN treatment at 25°C for 30 minutes effectively degrades the DNA. The required SAN concentration typically ranges between 50-1000 U/ml, depending on the extract and conditions. Verification of DNA removal can be done using agarose gel electrophoresis.
Application Background
