For reduction of viscosity in BugBuster lysates containing 0.5 M NaCl, add 25 U SAN per mL lysate (125 U/g wet weight of bacteria) and incubate at RT for 10-20 minutes with shaking.
If NaCl is not added to the lysate, more time or SAN is needed in order to reduce the viscosity. BugBuster lysate without NaCl may need 10x as much SAN as lysates containing 0.25 M NaCl.
Example: 1000 U per mL lysate without NaCl vs. 100 U per mL with 0.25 M NaCl.
Figure 1: SAN efficiently removes DNS from E. coli cell lysates