Use salt to your advantage and unlock new possibilities
INTRODUCING SAN HQ GMP
The Only GMP Grade
Salt Active Nuclease
We Solved the Paradox!
High Salt Conditions Now Boost, Not Hinder, DNA Clearing
The Salt & Nuclease Paradox: As salt concentration increases, conditions for DNA clearance improve, but enzyme function decreases. This “paradox” is resolved by SAN HQ GMP because its ability to clear DNA from viral vectors increases as salt concentration rises.
SAN HQ GMP performs best when conditions are optimal for clearing DNA. The results are maximum yield, purity, overall product quality, simplified workflow, reduced costs, and improved efficiency of DNA removal from your lysates in a single step.
At low, or no, salt concentrations, chromatin sticks to vectors causing aggregates that prevent enzymes from clearing DNA efficiently. At high salt concentrations, aggregates dissolve, so enzymes can clear DNA efficiently, minimizing residual DNA and maximizing yield. But high salt reduces the function of Benzonase and Denarase from ca. 50% at 150mM salt to near 0% at salt levels where DNA is most accessible for clearing.
Advantages of increased salt levels using SAN HQ GMP
By implementing a salt-resilient enzyme like SAN HQ GMP, you eliminate the necessity for desalting stages before and after nuclease treatment. This streamlines the bioprocessing workflow, resulting in time savings and potential cost reductions.
Research indicates that elevated salt conditions may boost the titer yield of AAV production. Operating under these conditions, a salt-resilient nuclease like SAN HQ GMP may contribute to an amplified virus yield.
Utilizing a salt-resilient enzyme like SAN HQ GMP can decrease enzyme usage, as it continues to function effectively under high salt levels. This could lead to cost savings and an increased efficiency of the process.
Advantages of minimized residual DNA in lysate
Reducing residual DNA in your lysate is crucial for both process efficiency and product yield. With SAN HQ GMP, you can achieve lower levels of residual DNA, resulting in multiple benefits for your operation.
Lower residual DNA levels enhance the final product's purity. This improvement could enhance the product's effectiveness and mitigate the risk of adverse immune responses in patients.
By aiding in residual DNA digestion, SAN HQ GMP ensures the final gene therapy product consists primarily of therapeutic viral vectors, leading to a more consistent and superior-quality product.
Efficient digestion of residual DNA may enhance the yield of the desired product. DNA binding to viral vectors can diminish the effective yield. By digesting this DNA, SAN HQ GMP can help maximize viral vector yield.
June 2023 DMF Filed
GMP processes Compliant
Q4 2023
SAN HQ GMP Ready for Use!
