To test our enzymes and generate performance data, ArcticZymes employ model systems, designed to mimic the real-life conditions in which we predict our enzymes will be used. Please note, the specific conditions of your own protocols may result in difference from our test data.
Salt Active Nuclease Performance Test Protocol
ArcticZymes Technologies’ salt performance test method uses complex, high molecular weight DNA, which is degraded to shorter fragments by nuclease treatment. The transition of high molecular weight DNA to smaller DNA fragments is measured by an increase in light absorbance at 260 nm (A260). Reactions are incubated 20 min at 37°C with 0.2 mg/ml Calf Thymus DNA, 5 mM MgCl₂, 25 mM Tris-HCl, pH 7.5 (@ 37°C) and various NaCl concentrations.
The reactions are stopped by adding perchloric acid, incubated on ice for 20 min followed by centrifugation. A260 is measured in the sample supernatants and values corrected against blank samples (no enzyme control). It is important to ensure that the amount of enzyme added to the assay gives a measurement within the linear range of the assay to get proportionality to the enzyme performance. Ideally, identical number of Units as defined by the manufacturers are used in each reaction. If different Unit amounts are tested, the A260 measurements are adjusted according to the Unit added to the assay.
If you have any questions about our model or testing parameters, please contact our application support team here.
