IsoPol® BST+
IsoPol® BST+ is a heat-tolerant Bst polymerase (large fragment) with enhanced strand-displacement activity. IsoPol® BST+ is active from 25 to 65°C. It is lacking 5’-3’- and 3’-5’-exonuclease activity.
Properties
Unit Definition: One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid insoluble material in 30 minutes at 65°C.
Strand-displacement activity: IsoPol® BST+ exhibits enhanced strand-displacement activity compared to Bst polymerase.
Figure 1: IsoPol® BST+ shows enhanced strand displacement activity compared to Bst polymerase. Activities at different concentrations of KCl at 37°C relative to activity at 50 mM KCl are shown for each polymerase.
Features: IsoPol® BST+ lacks 5’-3’- and 3’-5’-exonuclease activity.
Inactivation: IsoPol® BST+ is completely inactivated after one minute incubation at 95°C
Suggested reaction conditions: IsoPol® BST+ is active at pH 8.5 in buffer supplemented with 5-20 mM MgCl2 and 50-350 mM salt (NaCl or KCl), at 65°C.
Storage and Stability
The enzyme is stable at -20°C for 2 years in the supplied storage buffer. The enzyme tolerates at least four freeze-thaw cycles (-80°C) without loss of activity.
IsoPol® BST+ High Concentration Glycerol FREE
ArcticZymes offers IsoPol® BST+ High Concentration Glycerol FREE (IsoPol® BST+ HC Glycerol FREE) to ease the use of product development processes in sequencing technologies, solid phase amplification and several isothermal technologies.
Key features
- High concentration
- High purity
- Glycerol free for easy lyophilisation
Quality Control
ArcticZymes is dedicated to the quality of our products. IsoPol® BST+ is manufactured at our ISO 13485 certified facility in Norway.
dsDNA endonuclease activity
25 U IsoPol® BST+ was incubated with a supercoiled plasmid (1 µg) for 4 hours at 37°C. Agarose gel electrophoresis did not reveal any transformation of closed circular DNA to nicked DNA.
ssDNA endonuclease activity
25 U IsoPol® BST+ was incubated with M13 ssDNA (0.5 µg) for 4 hours at 37°C. Agarose gel electrophoresis did not reveal any visible signs of ssDNA degradation.
Additional Data and Information
We are pleased to provide additional data relating to IsoPol® BST+ upon request.
We appreciate suggestions for product modifications.
Disclaimer:
ArcticZymes Technologies ASA’s, including its subsidiaries, (“ArcticZymes”) products are intended for the further use of in manufacturing new products or research only. Certain applications of ArcticZymes’ products may require licenses from third parties. It is the express duty of any receiver of our products to acquire such licenses, if necessary. To the maximum extent allowed by law, ArcticZymes will not be accountable or liable for any damages, whether direct, indirect, incidental, or consequential, in connection with or arising from this document, including the use of it. ArcticZymes products may be covered by pending or issued patents, designs or design applications and/or trademarks or trademark applications or any other registered or unregistered Intellectual Property Right. For the avoidance of doubt, the General Conditions of ArcticZymes shall apply with respect to any and all purchases and use of the ArcticZymes products.