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Isothermal High Temperature Range

Isothermal High Temperature Range

IsoPol® BST⁺ For point-of-care diagnostic assays

Isothermal High Temperature Range

IsoPol® BST⁺ Isothermal High Temperature Range

OverView

These DNA polymerases are ideal for Isothermal Amplification

IsoPol® BST+ is a heat-tolerant BST polymerase (large fragment) with enhanced strand-displacement activity. IsoPol® BST+ is active from 25 to 65°C. It is lacking 5’-3’- and 3’-5’-exonuclease activity.

Key features

  • IsoPol® BST+ lacks 5’-3’- and 3’-5’-exonuclease activity.
  • Inactivation - IsoPol® BST+ is completely inactivated after one minute incubation at 95°C
  • Enhanced strand displacement and processivity.
  • Increased salt tolerance.

IsoPol BST+ Glycerol FREE 

Is the popular choice for isothermal applications such as LAMP and RT-LAMP at point-of-care diagnostics for its superior amplification performance and robustness. The enzyme is offered in a 1X storage buffer at a concentration < 300 U/µl

IsoPol BST+ High Concentration Glycerol FREE

(IsoPol BST+ HC Glycerol FREE) is used to ease the product development processes in sequencing technologies, solid phase amplification and several isothermal technologies. Same performance as IsoPol BST+ (i.e., high processivity, relative SDA, and relative activity) with the only difference that it is offered in a 2X storage buffer at a concentration > 500 U/

Key features

  • High concentration
  • High purity
  • Lyophilisation and automation friendly

Figures

Isothermal High Temperature Range - IsoPol® BST⁺ shows enhanced strand displacement activity
Figure 1. IsoPol® BST⁺ shows enhanced strand displacement activity
Isothermal High Temperature Range - IsoPol® BST⁺ shows enhanced strand displacement activity

IsoPol® BST+ shows enhanced strand displacement activity compared to Bst polymerase. Activities at different concentrations of KCl at 37°C relative to activity at 50 mM KCl are shown for each polymerase.

Isothermal High Temperature Range - IsoPol® BST⁺ tolerates higher concentrations of salt compared to Bst polymerase.
Figure 2. IsoPol® BST⁺ tolerates higher concentrations of salt compared to Bst polymerase.
Isothermal High Temperature Range - IsoPol® BST⁺ tolerates higher concentrations of salt compared to Bst polymerase.

IsoPol® BST+ tolerates higher concentrations of salt compared to Bst polymerase. Polymerase activity of Bst and IsoPol™ BST+ as function of NaCl data represented relative to highest activity of each polymerase.

Isothermal High Temperature Range - IsoPol BST⁺ has superior strand displacement activity
Figure 3. IsoPol BST⁺ has superior strand displacement activity
Isothermal High Temperature Range - IsoPol BST⁺ has superior strand displacement activity

IsoPol BST+ has superior strand displacement activity compared to Bst LF. SDA was measured at 37°C using a nicked dsDNA template.

Isothermal High Temperature Range - IsoPol BST⁺ has enhanced processivity compared to Bst LF
Figure 4. IsoPol BST⁺ has enhanced processivity compared to Bst LF
Isothermal High Temperature Range - IsoPol BST⁺ has enhanced processivity compared to Bst LF

IsoPol BST+ has enhanced processivity compared to Bst LF. Processivity was determined at 37°C using a primed circular ssDNA.

Isothermal High Temperature Range - IsoPol BST⁺ provides faster amplification in LAMP
Figure 5. IsoPol BST⁺ provides faster amplification in LAMP
Isothermal High Temperature Range - IsoPol BST⁺ provides faster amplification in LAMP
Isothermal High Temperature Range - IsoPol BST⁺ provides superior time-to-results in RT-LAMP
Figure 6. IsoPol BST⁺ provides superior time-to-results in RT-LAMP
Isothermal High Temperature Range - IsoPol BST⁺ provides superior time-to-results in RT-LAMP

IsoPol BST+ results in faster detection of target in both LAMP and RT-LAMP. When IsoPol BST+ was used in LAMP and RT-LAMP, time to results were strongly reduced compared to the non-engineered backbone, Bst LF. IsoPol BST⁺also significantly outperformed engineered versions of Bst LF from another vendor (Bst Vendor A). LAMP was used to detect 0.05 ng of λ DNA (Fig 5).RT-LAMP was performed with AMV RT to detect 10 ng of MS2 RNA template (Fig 6). LAMP and RT-LAMP were performed at 65°C.

Isothermal High Temperature Range - IsoPol BST⁺ tolerates serum
Figure 7. IsoPol BST⁺ tolerates serum
Isothermal High Temperature Range - IsoPol BST⁺ tolerates serum

IsoPol BST+ polymerase has high tolerance to matrix effects. LAMP was performed using λ DNA template in presence of human serum demonstrating that IsoPol BST+ maintains high activity over a wide range of serum concentrations. No amplification was detected in the absence of template.

Properties

Specifications
Unit Definition

One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid insoluble material in 30 minutes at 65°C.

Strand-displacement activity

IsoPol® BST+ exhibits enhanced strand-displacement activity compared to Bst polymerase.

Suggested reaction conditions

IsoPol® BST+ is active at pH 8.5 in buffer supplemented with 5-20 mM MgCl2 and 50-350 mM salt (NaCl or KCl), at 65°C.

Storage and Stability

IsoPol BST+ is stable at -20°C for up to 2 years in the supplied storage buffer. IsoPol BST+ Glycerol FREE and IsoPol BST+ High Concentration (HC) Glycerol FREE are stable at 4°C for up to 2 years in the supplied storage buffer.

Quality Control

ArcticZymes is dedicated to the quality of our products. IsoPol® BST⁺ is manufactured at our ISO 13485 certified facility in Norway.

Recommended Protocols

Ordering Info

Quantity
Product Name
SKU
Pack Size
Concentration
Price
1
T7 RNA Polymerase
74100-201
10 000 U
50 U/µl
$ 145.00 USD
133.00
145.00
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2
T7 RNA Polymerase
74100-110
100 kU
≥ 500 U/µl
$ 0.00 USD
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3
T7 RNA Polymerase
74100-100
As per request
As per request
$ 0.00 USD
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4
T7 RNA Polymerase Glycerol Free
74110-110
100 kU
≥ 500 U/µl
$ 0.00 USD
Request Quote
5
T7 RNA Polymerase Glycerol Free
74110-100
As per request
As per request
$ 0.00 USD
Request Quote
1
T4 DNA Ligase
71800-202
250 kU
5000 U/μl
$ 32.00 USD
30.00
32.00
Request Quote
1
AZscript RT
72100-201
10000 U
200 U/μl
$ 136.00 USD
123.00
136.00
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1
Heat&Run gDNA Removal Kit
80200-250
250 rxn
$ 651.00 USD
548.00
651.00
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1
M-SAN HQ ELISA Kit
70960-001
12x8 Strip plate
$ 756.00 USD
655.00
756.00
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1
HL-dsDNase Triton Free
70801-201
250 U
2 U/µl
$ 357.00 USD
305.00
357.00
Request Quote
1
PCR Decontamination Kit
80400-100
100 rxn
$ 130.00 USD
110.00
130.00
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1
AZtaq™ DNA Polymerase
73100-201
500 U
5 U/µl
$ 83.00 USD
75.00
83.00
Request Quote
1
HL-SAN Triton Free
70911-202
25000 U
25 U/µl
$ 560.00 USD
473.00
560.00
Request Quote
1
ArcticZymes Proteinase
71600-201
50 U
>200 U/ml
$ 155.00 USD
131.00
155.00
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1
IsoPol®
71500-201
200 U
5 U/µl
$ 75.00 USD
68.00
75.00
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1
ArcticZymes R2D Ligase
71900-105
5 kU
10 U/µl
$ 419.00 USD
361.00
419.00
Request Quote
1
rSAP
70700-201
1000 U
1 U/µl
$ 131.00 USD
116.00
131.00
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1
dsDNase Triton Free
70601-201
250 U
2 U/µl
$ 357.00 USD
305.00
357.00
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1
Cod UNG Triton Free
70501-201
100 U
1 U/µl
$ 130.00 USD
109.00
130.00
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1
IsoPol™ BST⁺
71502-202
1600 U
8 U/µl
$ 78.00 USD
71.00
78.00
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1
SAN High Quality
70920-150
500 kU
>250 U/µl
$ 5,767.00 USD
5011.00
5767.00
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1
HL-ExoI
70100-201
2500 U
>20 U/µl
$ 69.00 USD
65.00
69.00
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1
SAN HQ 2.0
70940-150
500 kU
250 U/µl
$ 5,767.00 USD
5011.00
5767.00
Request Quote
2
Cod UNG Triton Free
70501-202
1000 U
1 U/µl
$ 548.00 USD
456.00
548.00
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2
SAN High Quality
70920-160
5 MU
>250 U/µl
$ 21,913.00 USD
19035.00
21913.00
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2
PCR Decontamination Kit
80400-500
500 rxn
$ 519.00 USD
445.00
519.00
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2
HL-dsDNase Triton Free
70801-202
1000 U
2 U/µl
$ 1,055.00 USD
877.00
1055.00
Request Quote
2
IsoPol™ BST⁺
71502-120
10000 U
120 U/µl
$ 384.00 USD
350.00
384.00
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2
dsDNase Triton Free
70601-202
1000 U
2 U/µl
$ 1,055.00 USD
877.00
1055.00
Request Quote
2
T4 DNA Ligase
71800-100
as per request
as per request
$ 0.00 USD
Request Quote
2
ArcticZymes R2D Ligase
71900-100
As per request
As per request
$ 0.00 USD
Request Quote
2
M-SAN HQ
70950-120
200 kU
>250 U/µl
$ 3,155.00 USD
3024.00
3155.00
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2
IsoPol® SD+
71501-201
200 U
5 U/µl
$ 75.00 USD
68.00
75.00
Request Quote
2
HL-SAN Triton Free
70911-150
500 kU
25 U/µl
$ 5,425.00 USD
4714.00
5425.00
Request Quote
2
rSAP
70700-202
5000 U
1 U/µl
$ 519.00 USD
450.00
519.00
Request Quote
2
SAN HQ 2.0
70940-160
5 MU
>250 U/µl
$ 21,913.00 USD
19035.00
21913.00
Request Quote
2
Heat&Run gDNA Removal Kit
80200-50
50 rxn
$ 166.00 USD
139.00
166.00
Request Quote
2
HL-ExoI
70100-202
500 kU
>20 U/µl
$ 6,148.00 USD
5040.00
6148.00
Request Quote
2
AZscript RT
72100-100
As per request
As per request
$ 0.00 USD
Request Quote
2
ArcticZymes Proteinase
71600-110
1000 U
>200 U/ml
$ 1,419.00 USD
1188.00
1419.00
Request Quote
2
AZtaq™ DNA Polymerase
73100-110
5000 U
>50 U/µl
$ 619.00 USD
568.00
619.00
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3
Cod UNG Triton Free
70501-110
10 kU
1 U/µl
$ 3,920.00 USD
3472.00
3920.00
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3
SAN HQ 2.0
70940-202
25 kU
25 - 30 U/µl
$ 577.00 USD
498.00
577.00
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3
rSAP
70700-203
25 kU
1 U/µl
$ 1,965.00 USD
1703.00
1965.00
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3
HL-ExoI
70100-150
50 kU
>20 U/µl
$ 683.00 USD
593.00
683.00
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3
M-SAN HQ
70950-150
500 kU
>250 U/µl
$ 6,055.00 USD
5803.00
6055.00
Request Quote
3
ArcticZymes Proteinase
71600-100
As per request
>200 U/ml
$ 0.00 USD
Request Quote
3
IsoPol® BST⁺ Glycerol Free
71512-100
As per request
As per request
$ 0.00 USD
Request Quote
3
HL-dsDNase Triton Free
70801-203
2500 U
5 U/µ
$ 2,365.00 USD
1961.00
2365.00
Request Quote
3
AZtaq™ DNA Polymerase
73100-100
As per request
As per request
$ 0.00 USD
Request Quote
3
dsDNase Triton Free
70601-203
2500 U
5 U/µl
$ 2,365.00 USD
1961.00
2365.00
Request Quote
3
SAN High Quality
70920-202
25 kU
25-30 U/µl
$ 577.00 USD
498.00
577.00
Request Quote
3
HL-SAN
70910-202
25000 U
25 U/µl
$ 560.00 USD
473.00
560.00
Request Quote
4
IsoPol® BST⁺ Glycerol HC Free
71522-100
As per request
As per request
$ 0.00 USD
Request Quote
4
Cod UNG Triton Free
70501-151
50 kU
1 U/µl
$ 14,128.00 USD
12281.00
14128.00
Request Quote
4
ArcticZymes Proteinase Glycerol-FREE
71610-100
As per request
>200 U/ml
$ 0.00 USD
Request Quote
4
rSAP
70700-101
200 kU
1 U/µl
$ 13,375.00 USD
11631.00
13375.00
Request Quote
4
dsDNase Triton Free
70601-150
50 kU
50 U/µl
$ 17,069.00 USD
14830.00
17069.00
Request Quote
4
SAN HQ 2.0 ELISA Kit
70970-001
1x96 Well Plate
$ 755.00 USD
655.00
755.00
Request Quote
4
HL-dsDNase Triton Free
70801-110
100 kU
2 U/µl
$ 45,552.00 USD
39608.00
45552.00
Request Quote
4
HL-SAN
70910-150
500 kU
26 U/µl
$ 5,425.00 USD
4714.00
5425.00
Request Quote
4
SAN High Quality GMP
70980-150
500 kU
400-600 U/ul
$ 0.00 USD
Request Quote
5
Cod UNG Triton Free
70501-150
50 kU
50 U/µl
$ 14,128.00 USD
12281.00
14128.00
Request Quote
5
HL-dsDNase Glycerol-Free - Triton Free
70811-50
5000 U
>50 U/µl
$ 4,031.00 USD
3505.00
4031.00
Request Quote
5
rSAP Glycerol-free
70710-201
5000 U
>20 U/µl
$ 603.00 USD
521.00
603.00
Request Quote
4
M-SAN HQ
70950-155
1 MU
>250 U/µl
$ 10,905.00 USD
10450.00
10905.00
Request Quote
5
M-SAN HQ
70950-160
5 MU
>250 U/µl
$ 23,008.00 USD
22049.00
23008.00
Request Quote
5
dsDNase Triton Free
70601-110
100 kU
2 U/µl
$ 25,604.00 USD
22260.00
25604.00
Request Quote
5
SAN High Quality GMP
70980-160
5 MU
400-600 U/µl
$ 0.00 USD
Request Quote
6
rSAP Glycerol-free
70710-100
As per request
As per request
$ 0.00 USD
Request Quote
6
Cod UNG Glycerol-Free Triton Free
70511-105
5000 U
50 U/µl
$ 2,598.00 USD
2256.00
2598.00
Request Quote
6
dsDNase Glycerol Free, Triton Free
70611-50
5000 U
>50 U/µl
$ 4,031.00 USD
3505.00
4031.00
Request Quote
6
SAN High Quality Triton Free
70921-150
500 kU
>250 U/µl
$ 5,767.00 USD
5011.00
5767.00
Request Quote
6
M-SAN HQ
70950-202
25 kU
25-30 U/µl
$ 606.00 USD
580.00
606.00
Request Quote
6
HL-dsDNase
70800-201
250 U
2 U/µl
$ 357.00 USD
305.00
357.00
Request Quote
7
Cod UNG Glycerol-Free Triton Free
70511-150
50 kU
50 U/µl
$ 14,128.00 USD
12281.00
14128.00
Request Quote
7
SAN High Quality Triton Free
70921-160
5 MU
>250 U/µl
$ 21,913.00 USD
19035.00
21913.00
Request Quote
7
dsDNase
70600-201
250
2 U/µl
$ 357.00 USD
305.00
357.00
Request Quote
7
HL-dsDNase
70800-202
1000 U
2 U/µl
$ 1,055.00 USD
877.00
1055.00
Request Quote
8
SAN High Quality Triton Free
70921-202
25 kU
25-30 U/µl
$ 577.00 USD
498.00
577.00
Request Quote
8
Cod UNG
70500-201
100 U
1 U/µl
$ 130.00 USD
109.00
130.00
Request Quote
8
dsDNase
70600-202
1 000
2 U/µl
$ 1,055.00 USD
877.00
1055.00
Request Quote
8
HL-dsDNase
70800-203
2500 U
5 U/µl
$ 2,365.00 USD
1961.00
2365.00
Request Quote
9
Cod UNG
70500-202
1000 U
1 U/µl
$ 548.00 USD
456.00
548.00
Request Quote
9
SAN High Quality ELISA Kit
70930-001
1x96 Well Compact Plate Format
$ 756.00 USD
655.00
756.00
Request Quote
9
dsDNase
70600-203
2 500
5 U/µl
$ 2,365.00 USD
1961.00
2365.00
Request Quote
9
HL-dsDNase
70800-150
50 kU
50 U/µl
$ 30,330.00 USD
26368.00
30330.00
Request Quote
10
dsDNase
70600-150
50 kU
50 U/µl
$ 17,069.00 USD
14830.00
17069.00
Request Quote
10
Cod UNG
70500-110
10 kU
1 U/µl
$ 3,920.00 USD
3472.00
3920.00
Request Quote
10
HL-dsDNase
70800-110
100 kU
2 U/µl
$ 45,552.00 USD
39608.00
45552.00
Request Quote
11
dsDNase
70600-110
100 kU
2 U/µl
$ 25,604.00 USD
22260.00
25604.00
Request Quote
11
Cod UNG
70500-151
50 kU
1 U/µl
$ 14,128.00 USD
12281.00
14128.00
Request Quote
11
HL-dsDNase
70800-120
500 kU
2 U/µl
$ 170,682.00 USD
148412.00
170682.00
Request Quote
12
Cod UNG
70500-150
50 kU
50 U/µl
$ 14,128.00 USD
12281.00
14128.00
Request Quote
12
dsDNase Glycerol Free
70610-50
5000 U
80 - 140 U/µl
$ 4,031.00 USD
3505.00
4031.00
Request Quote
12
HL-dsDNase Glycerol-free
70810-50
5000 U
80 - 140 U/µl
$ 4,031.00 USD
3505.00
4031.00
Request Quote
13
Cod UNG Glycerol Free
70510-105
5000 U
50 U/µl
$ 2,598.00 USD
2256.00
2598.00
Request Quote
14
Cod UNG Glycerol Free
70510-150
50 kU
50 U/µl
$ 14,128.00 USD
12281.00
14128.00
Request Quote
Note: Article no. 71502-202 and 71502-120 are provided with IsoPol® BST+ 10X Buffer and MgSO4 .
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We don’t believe this will impact the overall layout but it may offer some opportunites for example adding a «Citations» button next to the ordering info. We’ll find out later in August as form the relationship

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FAQ's

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Isothermal High Temperature Range
SAN HQ
now available in
GMP grade