M-SAN High Quality nuclease (Bioprocessing Grade)

M-SAN HQ (Bioprocessing Grade) is the recent addition to our high salt tolerance nucleases portfolio. The biochemical properties of the SAN family of nucleases make them ideal for multiple bioprocessing and biomanufacturing workflows.

M-SAN HQ has been developed to offer the optimal solution for removal of nucleic acids at the near-physiological conditions used in many bioprocessing and biomanufacturing workflows. This novel, nonspecific endonuclease is active over a broad pH range and displays optimum activity at salt concentrations around 175 mM.

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High activity at physiological salt conditions

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No protease detected

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Supplied with extended product documentation

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High purity
(≥ 99%)

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Figure 1: Optimum activity in solutions at physiological salinity. M-SAN High Quality has optimum activity between 125 - 250 mM NaCl, which correlates to the salt content in buffers with physiological salinity. The activity was measured at 37°C in a 25 mM Tris-HCl buffer, pH 7.4, 2.5 mM MgCl2 and varying concentrations of NaCl. Maximum activity was set to 100%.
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Figure 2: Outperforms commonly used nucleases at physiological salinity. Physiological salinity is equivalent to approx. 150 mM NaCl. At this concentration of salt, M-SAN HQ is up to five-fold more efficient than commonly used nucleases with optimum performance in absence of salt. The activity was measured at 37°C in a 25 mM Tris-HCl buffer, pH 7.2, 5 mM MgCl2 and varying concentrations of NaCl. The same number of Units of each nuclease was used.
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Figure 3: Highly compatible with physiological pH. M-SAN HQ has optimal performance in the 7.2 - 8.7 pH range. This allows very high performance in most biological buffers. With a working range starting at pH 6.5, M-SAN HQ is also a candidate for DNA removal from insect cell media. The activity was measured at 37°C in a 25 mM Tris-HCl or Bis-Tris buffer at various pH, 2.5 mM MgCl2 and 150 mM NaCl. Activity measured at pH 7.7 was set to 100%.
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Figure 4: Superior performance at 37°C and above. M-SAN HQ is highly efficent for digesting DNA at 37°C, and tolerate temperatures up to 50°C. The activity was measured in a 25 mM Tris-HCl buffer at pH 7.8 (@measurement temperature), 5 mM MgCl2 and 150 mM of NaCl. 37°C was set to 100% as this is the temperature used for defining the activity in Units.

Properties

  • Source: Recombinantly produced in Pichia pastoris
  • Molecular weight: 24.5 kDa
  • Protein Purity: > 99% by SDS-PAGE analysis
  • Isoelectric point: 8.43
  • Unit definition: One unit is defined as the amount of enzyme that causes a ΔA260 = 1.0 in 30 minutes at 37° in
    25 mM Tris-HCl pH 7.6 (@25°C), 2.5 mM MgCl2, 150 mM NaCl, and 50 μg/ml calf thymus DNA.
  • Specificity: Nonspecific endonuclease cleaving single and double stranded DNA and RNA.
  • Working ranges:
    • Temperature: 20 – 50°C, optimal: 36 – 50°C
    • Salt concentrations (NaCI): 10 – 500 mM, optimal: 125 mM – 250 mM
    • Mg2+: > 0.5 mM is required for activity, optimal: 4 – 15 mM
    • pH: 6.5 – 9.5, optimal: 7.2 – 8.7

      Note: The working range is defined as above 10% activity and optimal range as above 80% activity.

  • Tolerance to typical buffer additives:
    • DTT and other reducing agents may inactivate M-SAN HQ
    • Urea: Not recommended
    • EDTA: Not recommended

M-SAN HQ ELISA Kit

ArcticZymes offers M-SAN HQ ELISA Kit to confirm the removal of M-SAN High Quality (Bioprocessing Grade) in bioprocessing and biomanufacturing applications.

Key features

  • Fast: 1.5 – 2 hours
  • Sensitive
    • Quantification range: 0.12 – 7.5 ng/ml
  • Accurate
    • 100% (±10%) Measuring range 0.47 – 7.50 ng/ml
    • 100% (±20%) Measuring range 0.12 – 0.47 ng/ml
  • Flexible: 96-well plate in 8-well strip format
Download M-SAN HQ brochure
Download M-SAN HQ ELISA Kit leaflet
Download M-SAN HQ poster