The kit contains HL-dsDNase which efficiently removes gDNA from RNA preps before running RT-qPCR.
- gDNA is removed, leaving RNA ready for reverse transcription in the same tube (Figure 1).
- Heat-labile dsDNase can easily be inactivated.
- This procedure minimizes pipetting steps and reduces hands-on time.
- The Heat&Run kit is especially well suited for high throughput experiments.
The high activity of the HL-dsDNase at low temperature and its heat-lability makes it ideal to use in a combination with a heat activated RT enzyme. The contaminating genomic DNA is cleaved at ambient temperature and increasing the temperature over 50°C will inactivate the DNase and start the Reverse Transcriptase.
Do you require gDNA removal in applications other than RT-qPCR? Contact our support team for assistance in implementing dsDNase treatment in your workflow.
Figure 1: Heat&Run protocol
- 10X reaction Buffer
- HL-dsDNase (50 reactions)
Storage Conditions: Store at -20˚C.
Sample Material: The Heat&Run kit is suited for gDNA removal of RNA in qPCR settings from samples with good RNA quality as some loss of RNA integrity can be expected.
Quality Control: The kit is tested for absence of RNase.